Innovat International Journal Of Medical & Pharmaceutical Sciences

CHEMICAL INVESTIGATION, ISOLATION AND BIOACTIVITY TESTING OF PHYTOMOLECULES FROM CYPERUS SCARIOSUS

2022-09-08T11:13:17+01:00

Objective: The present study was carried out to extract, isolate and evaluate the antimicrobial activity of the root and aerial part of Cyperus scariosus (Nagarmotha).

Material and Methods: In this research, cyperus oil was extracted from the root by various distillation techniques such as Water/hydro distillation (Clevenger apparatus), and Steam distillation (Boiler operated). The root and aerial part of Nagarmotha was extracted in different solvents such as hexane, aqueous, and ethanol and evaluated for antimicrobial activity at different concentrations against 6 gram-positive (Staphylococcus aureus, Streptococcus epidermidis, Bacillus subtilis, Yersinia enterocolitica, Salmonella typhimurium, Enterobacter aerogenes ), 1 gram-negative (E.Coli ) bacterial strains and 3 fungal strains (Candida albicans, Aspergillus niger, Aspergillus flavus ). Amikacin and fluconazole (100 μg/ml) were taken as standard drugs.

Result and Discussion: The % yield of oil was 0.84 % (v/w) by both methods. The Isolated compound was characterized by various spectroscopic techniques such as GC, GC-MS, and 1H NMR and established as (-)-ISOLONGIFOLEN-9-ONE. Root and aerial extract in different solvents showed mild antimicrobial activity at 100 μg/ml. Pure oil (NM Oil) showed mild to moderate antibacterial activity at 100 μg/ml against gram-positive bacteria but it was not shown any activity against gram-negative bacteria. Pure oil was also shown very good activity against fungal strains such as Candida albicans and Aspergillus flavus when compared with the standard drug Fluconazole.

Conclusion: The present study showed that the pure oil obtained from the Cyperus scariosus has the potential to diminish microbial growth and can be effective in the treatment of microbial diseases.

GLIOBLASTOMA. ASPECTS OF PATHOGENESIS AND THERAPEUTIC APPROACHES

2022-09-13T11:52:27+01:00

Glioblastoma is considered one of the most aggressive malignant brain tumors, which has an extremely unfavorable prognosis and low patient survival. The low sensitivity to standard multimodal therapy is explained by the heterogeneity of the tumor cell pool and the presence of glioblastoma stem-like cells in the tumor. In recent years, much attention has been paid to the identification and study of molecular genetic disorders of glioblastoma. The most significant of them are the IDH1 gene mutation and the MGMT methylation status. Currently, the gold standard of treatment is the use of neuronavigation and intraoperative tumor fluorescence in surgery, these methods allow the most accurate and complete removal of the tumor. In this article, we will consider the pathogenesis, experimental technologies and modern methods of treating this disease.

SPATIAL MEMORY ASSESSMENT IN RODENTS

2022-06-29T16:35:11+01:00

Stress may be acute, chronic and episodic. Acute and chronic stress may be differentiated on the basis of their onset of action and duration. Different animal models have been used to study and to evaluate the stress disorders including acute stress disorder and post-traumatic stress disorder. In most biomedical research rodents (rats and mice) are used. There are different apparatus ,which are used for the behaviour assessment, like plus-maze apparatus, zero-maze apparatus, T-maze apparatus etc..

SYNERGISTIC IMPACT OF HERBAL MEDICINE IN PEPTIC ULCER

2022-07-14T11:22:20+01:00

Up to 10% of people worldwide are affected by a chronic condition known as peptic ulcer. Peptic ulcer development is influenced by the pH of gastric juice and a decline in mucosal defences. Helicobacter pylori (H. pylori) infection and non-steroidal anti-inflammatory medicines (NSAIDs) are mucosal resistance to the damage being affected by two main elements. Conventional peptic ulcer therapy, Histamine-2 (H2) receptor antagonists, and proton pump inhibitors (PPIs) have shown negative consequences, relapses, and different medication combinations. Contrarily, therapeutic herbs and many illnesses may be prevented and treated with the help of their.