The present study aimed to evaluate the Anti-angiogenic activity of methanolic extract of Trianthema Portulacastrum Linn extract using Chick Chorioallantoic Membrane (CAM) model. Fertilized chicken eggs were incubated at 37 °C with 80% relative humidity and on day 2 of post incubation, 3 mL of albumin were withdrawn to minimize adhesion of the shell membrane with CAM. On 8th day, these eggs were divided into 5 groups of 10 each and treated with phosphate buffered saline and methanol extract of T. portulacastrum (METP) at concentrations of 50, 100, 200 and 300 μg/mL. After 48 h of incubation, CAM was cut around the disk and photos were taken and analyzed for numbers of vessel branch points contained in a circular region (equal to the area of each filter disk) were counted manually for the ability to inhibition angiogenesis. In the presence of METP, the number of blood vessels branches is reduced from 14 to 12, 9, 7 and 5, respectively to a concentration of 50, 100, 200 and 300 μg/mL and respectively an ability to inhibition angiogenesis to 14.29%, 35.71%, 50.00% and 64.29% with IC50 = 200 μg/mL. Based on these results, it was found that the methanolic extract of T. portulacastrum is capable of inhibiting the angiogenesis in Chick Chorioallantoic Membrane (CAM) Model.


Trianthema Portulacastrum Linn; Chick Chorioallantoic Membrane; CAM; Angiogenesis; Anti-angiogenesis activity; METP; Chicks

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