ANTIANGIOGENIC ACTIVITY OF TRIANTHEMA PORTULACASTRUM LINN EXTRACT USING CHICK CHORIOALLANTOIC MEMBRANE (CAM) MODEL

A. RAMA NARSIMHA REDDY, CH. SHANTHI, CH. HEMALATHA

Abstract


 

 The present study aimed to evaluate the Anti-angiogenic activity of methanolic extract of Trianthema Portulacastrum Linn extract using Chick Chorioallantoic Membrane (CAM) model. Fertilized chicken eggs were incubated at 37 °C with 80% relative humidity and on day 2 of post incubation, 3 mL of albumin were withdrawn to minimize adhesion of the shell membrane with CAM. On 8th day, these eggs were divided into 5 groups of 10 each and treated with phosphate buffered saline and methanol extract of T. portulacastrum (METP) at concentrations of 50, 100, 200 and 300 μg/mL. After 48 h of incubation, CAM was cut around the disk and photos were taken and analyzed for numbers of vessel branch points contained in a circular region (equal to the area of each filter disk) were counted manually for the ability to inhibition angiogenesis. In the presence of METP, the number of blood vessels branches is reduced from 14 to 12, 9, 7 and 5, respectively to a concentration of 50, 100, 200 and 300 μg/mL and respectively an ability to inhibition angiogenesis to 14.29%, 35.71%, 50.00% and 64.29% with IC50 = 200 μg/mL. Based on these results, it was found that the methanolic extract of T. portulacastrum is capable of inhibiting the angiogenesis in Chick Chorioallantoic Membrane (CAM) Model.


Keywords


Trianthema Portulacastrum Linn; Chick Chorioallantoic Membrane; CAM; Angiogenesis; Anti-angiogenesis activity; METP; Chicks

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References


Mandal A, Bishayee A. Trianthema portulacastrum Linn. Displays

anti-inflammatory responses during chemically induced rat

mammary tumorigenesis through simultaneous and differential

regulation of NF-κB and Nrf2 signaling pathways. Int J Mol Sci.

;16(2):2426-45. doi: 10.3390/ijms16022426, PMID 25622256.

Bhattacharya S, Chatterjee M. Inhibitory effect of Trianthema

portulacastrum L. diethylnitroso-amine-induced phenobarbital

promoted hepatocarcinogenesis. Neoplasma. 1999;46(2):105-11.

PMID 10466434.

Carmeliet P, Jain RK. Molecular mechanisms and clinical

applications of angiogenesis. Nature. 2011;473(7347):298-307. doi:

1038/nature10144, PMID 21593862.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal

Plants. New Delhi: Council of Scientific and Industrial Research;

Diniz C, Suliburska J, Ferreira IMPLVO. New insights into the

antiangiogenic and proangiogenic properties of dietary polyphenols.

Mol Nutr Food Res. 2017;61(6). doi: 10.1002/mnfr.201600912,

PMID 27981783.

Gacche RN, Meshram RJ. Angiogenic factors as potential drug

target: efficacy and limitations of anti-angiogenic therapy. Biochim

Biophys Acta. 2014;1846(1):161-79. doi:

1016/j.bbcan.2014.05.002, PMID 24836679.

Harborne JB. Phytochemical methods. London: Chapman & Hall;

Kokate CK. Practical pharmacognosy, Vallabh Prakashan, New

Delhi; 1994.

Narendra K, Rajendra S, Saminathan M, Singh KP, Dhama K, Arun

APM, Kurade NP. Therapeutic effect of hydroethanolic extract of

Trianthema portulacastrum L. against N-nitroso-N-Methylureainduced

mammary tumors in Wistar rats. Ind J Trad Knowl.

;19(2):406-15.

Ndong JDLC, Obiang CS, Ondo JP, Ndong-Atome GR, Abessolo

FO, Obame-Engoga LC. Ngoua-Meye-Misso RL. Clin Phytosci.

Phytochemical studies, Antiangiogenic, anti-inflammatory

and antioxidant activities of Scyphocephalium ochocoa Warb.

(Myristicaceae) Medicinal plant from Gabon;4:15-9.

Reddy ARNR, Mahender P, Reddy YN, Krishna RK.

Hypoglycemic and hypolipidemic activities of Trianthema

portulacastrum plant in normal and alloxan induced diabetic rats. Int

J Pharmacol. 2010;6(2):129-33.

Ribatti D. The chick embryo chorioallantoic membrane (CAM). A

multifaceted experimental model. Mech Dev. 2016;141:70-7. doi:

1016/j.mod.2016.05.003, PMID 27178379.

Ribatti D. Chick embryo chorioallantoic membrane as a useful tool

to study angiogenesis. Int Rev Cell Mol Biol. 2008;270:181-224.

doi: 10.1016/S1937-6448(08)01405-6, PMID 19081537.

Sarkar A, Pradhan S, Mukhopadhyay I, Bose SK, Roy S, Chatterjee

M. Inhibition of early DNA-damage and chromosomal aberrations

by Trianthema portulacastrum in carbon tetrachloride-induced

mouse liver damage. Cell Biol Int. 1999;23(10):703-8. doi:

1006/cbir.1999.0439, PMID 10736194.

Shibuya M. VEGF-VEGFR system as a target for suppressing

inflammation and other diseases. Endocr Metab Immune Disord

Drug Targets. 2015;15(2):135-44. doi:

2174/1871530315666150316121956, PMID 25772179.

Tufan AC, Satiroglu-Tufan NL. The chick embryo chorioallantoic

membrane as a model system for the study of tumor angiogenesis,

invasion and development of anti-angiogenic agents. Curr Cancer

Drug Targets. 2005;5(4):249-66. doi: 10.2174/1568009054064624,

PMID 15975046.


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