THE LIPID PEROXIDATION PROCESSES AFTER ADMINISTRATION OF NANOMODIFIED RESUSPENDING SOLUTION INTO ERYTHROCYTE-CONTAINING COMPONENTS OF THE DONOR

ANDREY BELOUSOV, TETIANA KALYNYCHENKO, ELENA MALYGON, MILITINA ANOSHYNA, MARYNA YAGOVDIK, VADIM YAVORSKIY, EKATERYNA BELOUSOVA

Abstract


 Objective: to improve the preservation of red blood cells containing donor blood components using NUS for resuspending. To achieve this goal, the first stage of the study examined the effect of a new resuspending solution (NUS) on lipoperoxidation, catalase activity, and red blood cell peroxidation resistance in donor blood components containing red blood cells.

Object of research: red blood cells from preservation blood of donors, which are intended for component transfusion therapy.

Materials and methods. The upgraded resuspending solution was obtained by treating saline sodium chloride with standardized magnetite nanoparticles (ICNB brand) using the Belousov's method with complete removal of NPs from the solution. Evaluation of the activity of lipid peroxidation (POL) processes was carried out by the spectrophotometric method's Volchegorsky. The optical density of each phase was measured on a Helios spectrophotometer. Statistical verification of the obtained data was carried out using computer programs STATISTICA 6.1.

Research and results. As a result of the study, the advantages of using NUS were established, namely: a significant decrease in the levels of primary and secondary lipid peroxidation products in the first 15 days of observation, an increase in catalase activity and resistance of red blood cells to peroxidation in the first stages of the study, inhibition of the shear reaction with neutral lipid peroxidation in the late stages of observation.

Conclusion. The saline sodium chloride solution which nanotechnologically upgraded by standardized biocompatible forms of NPs magnetite, has a positive effect on the ECC of donor blood when used as a resuspending agent. The advantage of exposure to this solution was a decrease in the intensity of POL reactions, an increase in catalase activity (CA) and red blood cells resistance (RBCR) on the first or second day after resuspending the erythrocytes were compared with a standard solution.

 


Keywords


nanomodified resuspending solution, oxidative homeostasis, peroxide resistance of red blood cells, components containing red blood cells, storage.

Full Text:

PDF

References


Smit Sibing CTh, Da PC, Taswell HF. E-book: quality assurance in blood banking and its clinical impact. - Martinus Nijhoff Publishers. USA: Boston. ISBN: 978-1-4613-2835-3; 1984.

Smit Sibinga CTh, Das PC, Loghem, van JJ, eds. Blood transfusion and problems of bleeding. ISBN: 90-247-3058-9; 1982.

Available from: http://www.nanolab.com.ua.

Patent of Ukraine №14817А UA A61N2/00 Method of production of a magnetic liquid for transport and retention of medicines in organism/A.N. Belousov № 96062463. Decl. 21.06.96; Publ. 18.02.97.5. Patent Agency of Ukraine No. 30538А UA A 23L 1/304 Therapeutic and preventive product MICROMAGE-B/Belousov № 98052704. 25.05.98. Publ. 15.11.00. Bul No. 6-11..

- 4 p. Patent of Ukraine №24322А UA A61N2/00 The sorbent for extracorporal detoxycation of biological liquids/A.N. Belousov. Bull. 1998;7.

- 3 p. Patent of Ukraine №24183А UA A61N2/00 The method extracorporal detoxycation of biological liquids/Belousov A.N. Bull. 1998;7.

Belousov AN. The use of magnetite nanoparticles in applied medicine. International Journal of Nano Dimension. 2011;2(1 (5), Summer):25-8. Print Edition ISSN: 2008-8868. Web Edition ISSN: 2228-5059.

Belousov AN. Investigation of the influence nanoparticles of magnetite controlled sorbent (MCS-B) on the functional activity of erythrocytes. Prospects Medicine and biology. LSMU. V. IY. Vol. 1; 2012 (ad). p. 94-7.

Belousov AN. Opening the mechanisms of cell regulation by nanotechnology preparations./7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers - Vancouver. Canadian. 2008:234-5.

Patent of Ukraine №31309А UA A61N2/00 Method of treatment diseases which connected with blood circulation disturbance/A.N. Belousov № 98084232. Decl. 04.08.98; Publ. 15.12.00.

Patent of Ukraine №42123А UA A61N2/00 Method of treatment diseases which connected with endocrine disturbance/A.N. Belousov № 98084233. Decl. 04.08.98; Publ. 15.10.01.

Patent of Ukraine 42132А UA A61N2/00 Method of treatment digestion system diseases/A.N. Belousov № 98084234. Decl. 04.08.98; Publ. 15.10.01.

Belousov AN, Voyda Y. Study of the reactions of microorganisms in response to the magnetite nanoparticles. OALib Journal is an all-in-one open access journal (ISSN Print: 2333-9721, ISSN Online: 2333-9705). 2015v;2:1-5.

Belousov AN. New prospects application of magnetite nanoparticles for the diagnosis of malignant tumors in MRI investigation. J Cell Mol Biol. 2017;2(003):1-4. ISSN: HJCM.

Belousov AN. The role of magnetite nanoparticles (ICNB) in discovery new factor which influence on permeability of erythrocytes and eryptosis. OALib. 2014;01(8):1-6. doi: 10.4236/oalib.1101055.

Belousov AN. Spectrum of application magnetite nanopaticles in medicine. Nanotech. 2009;2:154-7. - ISBN: 978-1-4398-1783-4 Chapter 3.

Belousov AN. Ultrastructure of hepatic cells in rabbits after injection of nanoparticles MCS-B. J Nanotechnol. 2013: Bio Sensors. Instruments, Medical Chapter 4. Environment and energy. In: Cancer nanotechnology & Nano medical sciences. Vol. 3 ISBN: 978-1-4665-6276-9. p. 258-60.

Belousov AN. Opening the mechanisms of cell regulation by nanotechnology preparations/7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers - Vancouver, Canada; 2008. p. 234-5.

Volchegorsky IA, Nalimov AG, Yarovinsky BG, Lifshits RI. [Comparison of different approaches to the determination of LP products in heptane-isopropanol blood extracts]. Quest Med Chem. 1989;1:127-31. Russian.

Anoshyna MYu, Kalynychenko TO, Gluhen’ka GT. [Evaluation of lipid peroxidation in samples of cryopreserved umbilical cord blood]. Ukr J Hematol Transfusiol. 2011;3:12-5. Ukrainian.

Balakhovsky SD, Balakhovsky IS. [Methods of chemical analysis of blood]. M Medgiz. 1953. 746 p. Russian.

[Laboratory Research methods in the clinic: handbook/ed. V. V], Menshikov et al. M: Medicine, 1987. 232 p. Russian.

Mavrichev AS, Bychkov GYu, Lysenko AA. No. Сopyright сertificate 1704083 RF, MKI. 02.08.89 ; publ. 07.01.92, Bul. No;01(33/50). [A method for assessing the peroxide resistance of erythrocytes] / N. A. Grigorovich:1. Russian. PMID 4726724/14; application No.

Rebrova OYu. [Statistical analysis of medical data. Application of the STATISTIS application software package]. Moscow: Mediasphere; 2006. 312 p. Russian.26. Petri A, Sabin K [Visual medical statistics: textbook. Manual]. from English. In: Leonov VP, editor / Translator. 3rd ed [reprint]. and additional M.: GEOTAR-Media; 2015. 216 p. Russian.

Grzhibovsky AM, Ivanov SI, Gorbatova MA. [Comparison of quantitative data of three or more paired samples using Statistica and SPSS software: parametric and nonparametric criteria]. Sci Healthc. 2016;5:5-29. Russian.

Rouchota M, Adamiano A, Iafisco M, Fragogeorgi E, Pilatis I, Doumont G, Boutry S, Catalucci D, Zacharioudaki A, Kagadis GC. Optimization of in vivo studies by combining planar dynamic and tomographic imaging: workflow evaluation on a superparamagnetic nanoparticles system. Mol Imaging. 2021 Jan 15;2021:6677847. doi: 10.1155/2021/6677847, PMID 33746630, PMCID PMC7953590.

Ribarič S. Nanotechnology therapy for Alzheimer’s disease memory impairment attenuation. Int J Mol Sci. 2021 Jan 22;22(3):1102. doi: 10.3390/ijms22031102, PMID 33499311, PMCID PMC7865945.

Javan Nikkhah S, Thompson D. Molecular modelling guided modulation of molecular shape and charge for design of smart self-assembled polymeric drug transporters. Pharmaceutics. 2021 Jan 22;13(2):141. doi: 10.3390/pharmaceutics13020141, PMID 33499130, PMCID PMC7912381.

Kang H, Mintri S, Menon AV, Lee HY, Choi HS, Kim J. Pharmacokinetics, pharmacodynamics and toxicology of theranostic nanoparticles. Nanoscale. 2015 Dec 7;7(45):18848-62. doi: 10.1039/c5nr05264e. PMID 26528835, PMCID PMC4648690.

Abaeva LF, Shumsky VI, Petritskaya EN, Rogatkin DA, Lubchenko PN. [Nanoparticles and nanotechnologies today and beyond]. Almanac Clin Med. 2010;22:10-6. Russian.

Nguyen KC, Rippstein P, Tayabali AF, Willmore WG. Mitochondrial toxicity of cadmium telluride quantum dot nanoparticles in mammalian hepatocytes. Toxicol Sci. 2015 Jul;146(1):31-42. doi: 10.1093/toxsci/kfv068. PMID 25809595, PMCID PMC4476459.

Belousov AN. Chapter 10. Application magnetite of nanoparticles (ICNB preparation) as magnetically-resonant contrasting means during visualization of tumours. Clean technology and sustainable industries organization; 2013. p. 379-81.


Refbacks

  • There are currently no refbacks.


© Copyright 2016. All Rights Reserved - Innovatpublisher CC BY Creative commons